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Sản Phẩm 
E.Z.N.A.® Gel Extraction Kit , Q-Spin
Gel purification of DNA is a common technique for the isolation of specific fragments from reaction mixtures. However, most methods either fail to completely remove agarose or shear DNA which can lead to problems in downstream manipulations. The E.Z.N.A.® Gel Extraction Kit uses proprietary chemistry and HiBind® technology to recover DNA fragments between 70 bp and 20 kb with yields exceeding 85%. The DNA band of interest is excised from the gel, dissolved in Binding Buffer, and transferred to a HiBind® DNA Mini Column. Following three rapid wash steps, DNA is eluted with the Elution Buffer and is ready for other applications. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
| Catalog No | Preps | Price |
|---|---|---|
| D2501-01 | 50 | Contact |
| D2501-02 | 200 | Contact |
Product Information
Illustrated Protocols
The E.Z.N.A.® Poly-Gel RNA Extraction Kit is a straight forward and simple system for recovering RNA probes from denaturing acrylamide gels. After the in vitro transcription reaction, the product is run on a denaturing polyacrylamide gel (a mini protein gel apparatus can be used) to separate the DNA template, full-length RNA probe, any prematurely terminated products and free-nucleotides, by size. The gel is exposed to film and stained or UV shadowed (depending on the nature and quantity of the probe made, e.g. radioactive, non-isotopic or unlabeled probe). Full-length probe is identified and the band is cut from the gel. The probe is eluted by passive diffusion from the gel fragment and further purified using an HiBind® RNA spin-column. The procedure can produce enough probe, ready for hybridization in just 1-4 hours.
Illustrated Protocols
Product Information
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